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with all-in-one library preparation kits
→ Our new CORALL RNA-Seq V2 Library Prep Kits
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RNA Sequencing
(noun) /RNA ˈsiːkwənsɪŋ/
Whole-transcriptome analysis with RNA sequencing (RNA-Seq) is at the cutting edge of NGS capabilities and allows to determine RNA molecules in a sample at the moment of sampling. The transcriptome is a highly dynamic cellular feature that opens up a world of discovery potential. Changes in response to drugs, various states of disease, post-transcriptional modifications, and alternatively spliced transcripts are just some examples of discoveries made possible by RNA-Seq.
RNA-Seq Applications
RNA-Seq is used to measure gene and transcript abundance levels, and to assess qualitative information of the transcriptome of all species. It is commonly used to:
- perform differential expression analysis,
- identify and quantify transcript isoforms,
- perform alternative splicing studies,
- assess long non-coding RNAs,
- detect transcript variants (including SNPs and SNVs),
- study fusion transcripts,
- (re-)annotate model and non-model organisms,
- perform transcriptomics on archived biobank samples (FFPE material)
CORALL RNA-Seq Library Prep Kits
The CORALL RNA-Seq Library Prep Kits enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries for whole transcriptome analyses using Illumina® NGS platforms.
Watch a short video to learn more about CORALL:
Benefits
One for all and all-in-one. CORALL RNA-Seq is a well-established, fragmentation-free, and universal whole transcriptome RNA-Seq library prep which is completed in 6 steps (4.5 hours). CORALL is the universal solution for all your samples offering exceptional performance on low input samples, down to 1 ng starting amount. It is the ideal solution for total RNA-Seq analysis on degraded and FFPE samples.
New! Adjustable library size to perfectly fit your application: CORALL RNA-Seq V2 allows generation of longer libraries (~ 550 bp) for demanding applications such as alternative splicing, isoform analysis, and fusion detection.
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Time saving
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Excels with low input
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Unlocks challenging samples
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NEW! Adjustable size
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Not sure which CORALL RNA-Seq product is right for you?
Try the CORALL Configurator and find the ideal kit for your application
in less than 2 minutes!
NEW! CORALL RNA-Seq V2 Specifications
CORALL RNA-Seq V2 is a fast and flexible kit for whole transcriptome sequencing with a wide input range. CORALL RNA-Seq V2 is available as standalone kit, mRNA-Seq bundle including poly(A) selection and Total RNA-Seq bundle including RiboCop rRNA depletion (Table 1).
Table 1 | CORALL RNA-Seq V2 workflow specifications | ||||||||
---|---|---|---|---|---|---|---|---|
Feature | Specification | Benefit | ||||||
CORALL RNA-Seq V2 (stand-alone Kit) | CORALL mRNA-Seq V2 with Poly(A) Selection | CORALL Total RNA-Seq V2 with RiboCop | ||||||
Input RNA quantity | 0.1 ng – 100 ng | 1 ng – 1000 ng | 1 ng – 1000 ng | Supports the widest input range available on the market, delivers consistent library output over the full input range | ||||
RNA types | Total RNA | Poly(A) RNA | Ribo-depleted RNA | Supports all Whole Transcriptome RNA-Seq Applications | ||||
RNA quality | All qualities, including degraded RNA, FFPE | High-quality RNA (RIN > 8) | All qualities, including degraded RNA, FFPE | Supports all RNA qualities, high sensitivity and high-quality results for difficult material and FFPE samples | ||||
Workflow time* | 4.5 hours | 5.5 hours | 6 hours | Time-saving workflow with less steps, from sample to sequencing-ready library in one day | ||||
Kit reaction sizes | 24, 96, 384 | 96, 384 | 24, 96 | Scalable for evaluation, adoption and high-throughout applications | ||||
Technology | Fragmentation-free library prep using displacement-stop technology | Poly(A) selection by hybridization / capture and CORALL library prep | Hybridization / capture rRNA-depletion without enzymatic digestion and CORALL library prep | – Fragmentation-free workflow – No second strand synthesis – No adaptor titration, less adapter-dimers – Excellent inherent strandedness (> 99 %) | ||||
Unique Dual Indexing (UDI) | All CORALL RNA-Seq V2 Kits and Bundles contain 12 nt UDI Sets. | Maximized sequencing output on all instruments | ||||||
Unique Molecular Identifiers (UMIs) | All CORALL RNA-Seq V2 Kits and Bundles contain built-in UMIs. | Supports identification and removal of PCR duplicates for all sample types and quantities without additional purchases | ||||||
Automation Capability | All CORALL RNA-Seq V2 workflows can be automated on liquid handlers. | Supports high-throughput applications | ||||||
* Workflow time is based upon incubation times and expected times for hands-on-steps. Actual workflow time may vary. | ||||||||