Do you have any questions?
Is LUTHOR HD the right solution for your RNA research? Let’s find out!
LUTHOR single cell dispenser
Complete workflow
Low cell input
Compact size
Keep track of your cells
LUTHOR single cell dispenser efficiently isolates a full plate of individual cells in only 10 minutes by dispensing them as gently as manual pipetting.
The core technology of the LUTHOR single cell dispenser is based on impedance measurement of cells passing through the proprietary dispensing tip which records the electrical signal. This allows to monitor that only one cell is dispensed per well and directly identifies doublets and debris which can be excluded from further analysis.
A dedicated, intuitive, software is provided free of charge with the instrument to ensure seamless operation and streamlined analysis.
Instrument Specifications and Compatibilities
General Specifications
General Specifications | ||
---|---|---|
Power Supply | External, 12 V, 11.5 A, 138W | |
Plate holder | 2 plates (96 or 384 wells) | |
222.4x96 | 96-well plate | |
223.24 | 384-well plate (customized setup)* | |
Minimal cell number | 100 cells** | |
Calibration | Not required | |
Cleaning | Not required | |
Sample prep. (time to dispense) | 10 min (96-well plate) | |
40 min (384-well plate) | ||
Cell parameters | Size, doublets | |
Input voltage (External power supply) | 100 - 240 V~, 50 - 60 Hz | |
Input current (External power supply) | max 2.0 A | |
Input voltage (Instrument) | 12.0 V | |
Max. power (Instrument) | 40 W | |
Well plates compatibility | 96-well plate: non-skirted, semi-skirted (with plate holder) | |
96-well plate: fully skirted (without plate holder) | ||
384-well plate: fully skirted (without plate holder) | ||
Any 96-well and 384-well plate that meets ANSI/SLAS 1-2004 through ANSI/SLAS 4-2004 standards is compatible. | ||
Loading tube | Sarstedt 0.5 ml, 150 ml | |
Loading volume | per 30s load. time @1.5x10⁴ cells/mL : 10µL, 200 cells | |
Dispensing volume (receiving well) | Down to 5 ml | |
Particle range detection capability | Dielectric, from 8 µm to 25 µm | |
*contact: support@lexogen.com | *contact: support@lexogen.com | |
** LUTHOR single cell dispenser can be adapted down to 100 cells as initial input. Preset programs are readily available, and if needed, Lexogen offers additional support to customize new programs tailored to each experimental setup. | ** LUTHOR single cell dispenser can be adapted down to 100 cells as initial input. Preset programs are readily available, and if needed, Lexogen offers additional support to customize new programs tailored to each experimental setup. | |
Compressed Air Specifications
Compressed Air Specifications | |
---|---|
Minimum operating pressure | Less than 0.2 psi |
Environmental Conditions
Environmental Conditions | |
---|---|
Temperature | 5 °C to 40 °C (41 °F to 104 °F) |
Maximum relative humidity | 80% for temperature up to 31 °C |
Instrument Measurements
Instrument Measurements | |
---|---|
Instrument weight | 11.1 kg |
Instrument Size | 328 x 271.5 x 369 mm |
Additional Hardware
Additional Hardware | |
---|---|
Computer screen with HDMI cable | Required, not included |
Keyboard | Required, not included |
Mouse | Required, not included |
Consumables
Consumables | |
---|---|
LUTHOR single cell dispensing Kit | Cat. No. 231.04, sufficient for 4 dispensing experiments |
LUTHOR single cell dispensing Kit
The LUTHOR single cell dispensing Kit (Cat. No. 231.04) contains reagents and dispensing tips sufficient for four experiments. The consumables kit is required for use of the instrument.
Compatible RNA-Seq library preparation kits for NGS experiments from single-cells
Obtaining the LUTHOR single cell dispenser for your lab
Performance
Consistent Dispensing Accuracy – Even with Initial Low Cell Numbers
Fresh or Fixed: Consistent Library Quality
Figure 2 | Comparative analysis between fresh and fixed DU145 cells.
Upper panel: Gene detection rates shown as average number of genes detected at increasing read depth. 8 single DU145 cells were analyzed from dispensing experiments using suspensions of A) fresh and B) fixed cells. Libraries for high-definition scRNA-Seq were generated with the LUTHOR HD Library Prep Kit and sequenced on Element’s AVITI platform. Gene detection was monitored at a threshold of ≥ 1 CPM (Counts Per Million). Read depth is expressed as raw reads. Standard deviation is indicated in light green.
Lower panel: Representative correlation analyses (orthogonal regression of gene-based collapsed and mapped reads) obtained from one fresh cell and one fixed cell. The R² value was computed from C) 28 (fresh vs. fresh), D) 28 (fixed vs. fixed) or E) 64 combinations (fresh vs. fixed) of single cells (sample size: 8 cells per condition).
For more information on the Fixation protocol – see our FAQs.
Excellent Alternative to Flow Cytometry-assisted Cell Dispensing
Figure 3 | Comparison of read statistics from HEK293T cells isolated with the LUTHOR single cell dispenser and by flow cytometry. A) Percentage of total mapped reads and reads on genes. B) Percentage of reads mapping uniquely, multi-mapping and unmapped reads. C) Percentage of exonic*, intronic*, and intergenic reads*. D) Percentage of protein coding**, mitochondrial ribosomal RNA**, and cytoplasmic ribosomal RNA*. Cell input was 10,000 cells (at 20 cells/ml) for LUTHOR single cell dispenser and 1 million cells (at 1,000 cells/ ml) for the flow cytometer (BD FACSMelody, with 100-mm nozzle and 34,000 drops/sec). scRNA-Seq libraries were prepared with the LUTHOR HD Library Prep Kit. 6 samples per isolation method were analyzed at 1 M reads / sample.
*based on mapped reads; **based on exonic reads.
Unsurpassed Gene Detection Sensitivity and Precision with Both Flow Cytometry-assisted and LUTHOR single cell dispenser-assisted Cell Isolation
Figure 4 | Comparative analysis between HEK293T cells isolated with the LUTHOR single cell dispenser and by flow cytometry.
Upper panel: Gene detection rates shown as average number of genes detected at increasing read depth. 6 single HEK293T cells isolated with A) LUTHOR single cell dispenser and B) a flow cytometer. Libraries for high-definition scRNA-Seq were generated with the LUTHOR HD Library Prep Kit and sequenced on Illumina’s NextSeq2000 platform. Gene detection was monitored at a threshold of ≥ 1 CPM (Counts Per Million). Read depth is expressed as raw reads. Standard deviation is indicated in light green.
Lower panel: Representative correlation analyses (orthogonal regression of gene-based collapsed and mapped reads) obtained from single HEK293T cells isolated with either the LUTHOR single cell dispenser or a flow cytometer. The R2 value was computed from C) 15 (LUTHOR dispenser replicates), D) 15 (flow cytometer replicates) or E) 36 combinations (LUTHOR dispenser vs. flow cytometer) of single cells (sample size: 6 cells per condition). Cell input was 10,000 cells (at 20 cells/ml) for LUTHOR single cell dispenser and and 1 million cells (at 1,000 cells/ ml) for the flow cytometer (BD FACSMelody, with 100-mm nozzle and 34,000 drops/sec).
Workflow
FAQ
Frequently Asked Questions
Access our frequently asked question (FAQ) resources via the buttons below.
Please also check our General Guidelines and FAQ resources!
How do you like the new online FAQ resource? Please share your feedback with us!
Downloads
LUTHOR single cell dispenser
Safety Data Sheet
If you need more information about our products, please contact us through support@lexogen.com or directly under +43 1 345 1212-41.
Cat. № | Product Name |
---|---|
231.01 | LUTHOR single cell dispenser |
231.04 | LUTHOR single cell dispensing Kit, 4 dispensing experiments |