Lexogen Indexing Solutions
Lexogen’s Indexing Solutions
Information:
Single indexing = one index (i7) per library
Dual indexing:
- combinatorial (also called CDI for Combinatorial Dual Indexing) = two indices (i5 and i7) on each end of the insert, in different combinations. Each i5 and each i7 can be used several times (but each combination is unique).
- unique (also called UDI for Unique Dual Indexing) = two indices (i5 and i7) on each end of the insert, in different combinations. Each i5 and i7 is unique (you will never find the same i5 or i7 index twice in a pair).
Lexogen offers single indexing, dual indexing and unique dual indexing for all needs.
Prevalent indexing solutions as part of Lexogen’s library prep kits:
QuantSeq | CORALL | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Indexing | rxn | i7 6 nt | i5 6nt | UDI 12nt | Cat. No. | i7 6nt | UDI 12nt | Cat. No. | ||||
Single | 24 | 24 | 015.24 | 24 | 095.24 | |||||||
96 | 96 | 015.96 | 96 | 095.96 | ||||||||
192 | 2x96 | 015.2x96 | ||||||||||
Dual | Combinatorial | 384 | 96 | 4 | 015.384 | |||||||
Unique V2 (recommended) | 24 | 24 | 191.24 (A1), 192.24 (B1) | 171.24 (A1), 175.24 (B1) | ||||||||
96 | 96 | 191.96 (A1), 192.96 (B1), 194.96 (A2), 195.96 (A3), 196.96 (A4) | 171.96 (A1), 175.96 (B1), 172.96 (A2), 173.96 (A3), 174.96 (A4) | |||||||||
384 | 384 | 193.384 | 176.384 | |||||||||
Unique V1 | 96 | 96 | 113.96 (A1), 115.96 (B1), 129.96 (A2), 130.96 (A3), 131.96 (A4) | 96 | 117.96 (A1), 118.96 (B1), 132.96 (A2), 133.96 (A3), 134.96 (A4) | |||||||
384 | 384 | 115.384 | 384 | 119.384 | ||||||||
Flexible dual indexing solutions available as Add-on kits to be combined with library prep kits:
Number of indices included | Cat. No. | |||||||
---|---|---|---|---|---|---|---|---|
Indexing | Possible index combinations | i7 6nt (included in Lexogen’s library prep kits) | i5 6nt (Add-on kits) | UDI 12nt (Add-on kits) | i5 6nt Dual Indexing Add-on Kit3 | 12nt UDI Add-on Kit3 (recommended) | QuantSeq (i7 6nt indices included) | CORALL (i7 6nt indices included) |
Dual (combinatorial) | 96 | 24 | 4 | 047.4x96¹ | 015.24 | 095.24 | ||
384 | 96 | 4 | 047.4x96¹ | 015.96 | 095.96 | |||
9,216 | 96 | 96 | 047.96 | 015.96 | 095.96 | |||
Unique Dual | 96 | 96 | 96 | 047.96 | 015.96 | 095.96 | ||
96 | (96)² | 96 pairs | 198.96 (A1), 199.96 (A2), 200.96 (A3), 201.96 (A4), 202.96 (B1) | 015.96 | 095.96 | |||
384 | (96)² | 384 pairs | 203.384 | 015.384⁴ | 4x 095.96 | |||
Notes:
1 module 047.4×96 provides enough i5 primers for 4×96 reactions
2 these primers will be discarded, and replaced with UDI
3 the PCR mix of the Add-on kit must be used instead of the one provided in the QuantSeq or CORALL kit
4 i5 indices will be discarded, and replaced with UDI
The Indexing Add-on kits are further described below.
Unique Dual Indexing with 8, 10, or 12 nt read-out (recommended)
- Lexogen UDI 12nt UDI Add-on V2 Kits for unique dual indexing of up to 96 (Cat. No. 198.96 to 202.96) and 384 (Cat. No. 203.384) samples, with improved robustness. These kits are compatible with existing CORALL and QuantSeq kits, but also library preparation kits from other vendors.
- The V1 Add-on kits (107.96, 108.96, 109.96, 110.96, 111.96 and 120.384) are still available for purchase for a limited period
- Add-on kits include the PCR amplification mastermix (UDI Sets are available as a standalone product with Cat. No. 101-105 and 156)
Dual Indexing with 6 nt read-out
To be used with the i7 6 nt Index Set provided in the library preparation kit (QuantSeq FWD 015 or CORALL 095)
- Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) for dual indexing of up to 96 (4×24) samples (Cat. No. 047.4×24)
- Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) for dual indexing of up to 384 (4×96) samples (Cat. No. 047.4×96)
- Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096) for dual indexing of up to 9,216 (96×96) samples* (Cat. No. 047.96)
Unique Dual Indexing with 6 nt read-out
- Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096) for unique dual indexing of up to 96 samples (Cat. No. 047.96)
Single Indexing with 6 nt read-out
These indices are already provided in QuantSeq and CORALL kits 015 and 095. They can be purchased separately for other purposes, or as a replacement set.
- Lexogen i7 6 nt Index Set (7001-7096) for single indexing of up to 96 samples (Cat. No. 044.96)