Description
The SLAMseq Explorer Kit – S4U Incorporation Module (Cat. No. 060) is used to measure the efficiency of S4U incorporation into newly synthesized RNA. The kit enables labeling of cells with 4-Thiouridine (S4U) and provides additional reagents required for stabilizing S4U-labeled RNA during RNA isolation, digestion of RNA to single nucleosides, and standards for HPLC analysis. It is compatible for reverse phase HPLC analysis.
The kit is sufficient for analysis of S4U incorporation in 24 samples in total. SLAMseq is compatible for cell suspensions, adherent cells, and 3D-scaffold cell cultures.
No access to an HPLC? We recommend measuring the efficiency of S4U incorporation by 3’ mRNA sequencing with QuantSeq (227.24 SLAMseq Explorer Kits for 3’ mRNA-Seq with QuantSeq FWD V2, 24 preps).
Features and Application:
- Label newly synthesized RNA with S4U
- Prepare total RNA for HPLC analysis
- Measure S4U uptake and incorporation rates using HPLC analysis
Compatibility information:
- Cell suspensions
- Adherent cells
- 3D-scaffold cell cultures
- 24-well format (up-scaling and down-scaling of culture volumes is possible)
- Reverse phase HPLC analyses
Kit Content:
- Reagent Box
- User Guide
